Yes, you can use this product along both the upper and lower lash line.Related ArticlesCan The Eyebrow and Lash Enhancing Serum be used under makeup?How long does The Eyebrow and Lash Enhancing Serum take to dry?Can The Eyebrow and Lash Enhancing Serum be used after procedures like brow lamination or lash lift?Can I use The Eyebrow and Lash Enhancing Serum on my lower lash?Can I still wear my individual lashes whilst using The Eyebrow and Lash Enhancing Serum?